Journal: Nucleic Acids Research

Article Title: A conserved nutrient responsive axis mediates autophagic degradation of miRNA–mRNA hybrids in blood cell progenitors

doi: 10.1093/nar/gkad1047

Figure Lengend Snippet: Targeting miRNAs and amino acid sensing in Drosophila blood cell progenitors. ( A–A' ) The primary lobe of the lymph gland was isolated from a 48 h AEH larvae by laser capture micro-dissection (LCM) technique. ( B ) Marker analysis was done after cDNA conversion of RNA isolates post-LCM dissection of the primary lobes, which is then visualized through Agarose gel electrophoresis. ( C ) Heatmap depicting the expression profile of the Drosophila miRNAs present in the primary lobe (48 h AEH) retrieved through LCM. ( D–F' ) EdU incorporation analysis of lymph glands at (D, D') 36 h, ( E, E' ) 48 h and ( F, F' ) 60 h upon Dicer1 down regulation from the progenitor. ( G–G' ) Volumetric comparison by 3D-reconstruction of progenitors in the wildtype ( G ) and ( G' ) Dcr1 knockdown lymph gland. (H–K) Quantitative time-kinetic analysis of Wildtype and Dcr1KD progenitors depicting the ( H ) proliferation rate, ( I ) progenitor number, ( J ) progenitor volume and ( K ) individual progenitor cell volume observed at 36, 48 and 60 h AEH respectively. ( L–N' ) Quantitative analysis of progenitor number and volume upon increasing concentration of Carbon sources. ( L–L' ) in excess Carbohydrates, ( M–M' ) in excess lipids, ( N–N' ) in excess proteins. Scale, 20 μm in all images. Individual dots represent ‘ n ’ of the sample. Significance was evaluated using two-way ANOVA with Tukey's test was performed for grouped analyses. Error bar: standard deviation (SD). Data are mean ± SD. * P < 0.033, ** P < 0.002 and *** P < 0.001. See also .

Article Snippet: Primary Bone Marrow CD34+ Hematopoietic Cells, Normal, Human were acquired from ATCC (PCS-800-012).

Techniques: Isolation, Dissection, Marker, Agarose Gel Electrophoresis, Expressing, Comparison, Knockdown, Concentration Assay, Standard Deviation

Journal: Nucleic Acids Research

Article Title: A conserved nutrient responsive axis mediates autophagic degradation of miRNA–mRNA hybrids in blood cell progenitors

doi: 10.1093/nar/gkad1047

Figure Lengend Snippet: Human blood progenitors exhibit leucine responsive proliferation similar to Drosophila . ( A–A' ) EdU incorporation analysis of CD34+ CD10-Lin- myeloid biased HSPC in undeprived and leucine-deficient media. ( B and B' ) Quantitative analysis in the proliferation rate of CD34+ CD10-Lin- HSPCs and KG1 cells. ( C–D' ) Dose-dependent recovery in the proliferation rate of CD34+ CD10-Lin– HSPC upon increasing concentration of leucine in leucine deprived media. ( D–D' ) Quantitative analysis of the recovery in proliferation index of CD34+ CD10-Lin- HSPCs and KG1 cells. ( E ) OPP incorporation rate analysis of CD34+ CD10-Lin-myeloid biased HSPC in early stages (6 h) of leucine deprivation versus late stages (16 h). ( F ) Lysotracker staining of acidic autolysosome upon leucine starvation at an early time point in HSPCs. ( G ) Cyto-ID staining of autophagosomes in response to leucine starvation at early time point in HSPCs. ( H ) Quantitative analysis of OPP incorporation rate at 6 and 16 h of leucine starvation condition in HSPCs. ( I ) Quantitative measurement of increased acidic vesicles (Lysotracker) in HSPCs. ( J ) Quantitative measurement of autophagosomes (Cyto-ID) in HSPCs. Scale, 20 μm in all images. Individual dots represent ‘n’ of the sample. Two-way ANOVA was performed for grouped analyses. One-way ANOVA was performed for individual multiple comparisons. Error bar: standard deviation (SD). Data are mean ± SD. * P < 0.033, ** P < 0.002 and *** P < 0.001. See also .

Article Snippet: Primary Bone Marrow CD34+ Hematopoietic Cells, Normal, Human were acquired from ATCC (PCS-800-012).

Techniques: Concentration Assay, Staining, Standard Deviation

Journal: Nucleic Acids Research

Article Title: A conserved nutrient responsive axis mediates autophagic degradation of miRNA–mRNA hybrids in blood cell progenitors

doi: 10.1093/nar/gkad1047

Figure Lengend Snippet: Leucine-based proliferation of human blood progenitors also targets autophagy-dependent miRNA turnover. ( A ) Heatmap representing high throughput transcriptomic analysis at 6 h pulse of leucine starvation in CD34+ CD10-Lin– HPSCs. ( B ) Network map depicting pathway characterization of the differentially expressing genes in CD34+ CD10-Lin– HSPC as a response to leucine deprivation. ( B' ) GO analysis of the most significantly affected biological process in CD34+ CD10-Lin- HSPCs as a response to leucine deprivation. ( C ) Western blot depicting reduction in Ago2 protein upon early and late response to leucine starvation. ( D ) Levels of p62/SQSTM1 show a similar reduction as in (C). ( E ) Co-immunoprecipitation depicting physical interaction between p62 and Ago2. The blot depicts a basal level of Ago2 interaction that increases upon leucine deprivation (Low exposure of the same blot is in ). ( F ) EdU incorporation analysis upon leucine starvation and simultaneous treatment with BafilomycinA1 (BafA1). ( F' ) Quantitative analysis on the proliferation rate upon leucine deprivation and BafA1 treatment. ( G ) Levels of Ago-2 upon leucine deprivation and BafA1 treatment detected by western blotting. ( H ) Quantitative analysis of Ago2 protein levels upon leucine deprivation and BafA1 treatment. ( I ) Schematic representing miRNA-9 sponge construct used for RNA-IP (above). Destabilized GFP transcript with deleted sponge sites (served as Control-Sponge) and destabilized GFP transcript with 8x sponge sites (miRNA-9 Sponge) (below). ( J ) Agarose gel demonstrates a qualitative assessment of miRNA-9 sponge enrichment upon p62-RNA-IP. ( J' ) A qPCR-based analysis of Control-sponge and miRNA-9 sponge enrichment upon p62-RNA-IP and assessment of an endogenous transcript (actin) within the pulled fraction. ( K ) qPCR analysis of RNA-IP done with p62 as bait using Control-sponge and miRNA-9 sponge construct upon leucine deprivation (left). qPCR analysis of puromycin transcript acts as a negative control (right). ( K' ) qPCR analysis of RNA-IP done with p62 on miRNA-9 sponge construct upon leucine deprivation and BafA1 treatment during leucine deprivation (left). A qPCR analysis of GFP in the Input fraction of the same (right). Scale, 20 μm in all images. Individual dots represent ‘n’ of the sample. Multiple t -test with Welch correction was performed for RNA-IP analysis. One-way ANOVA was performed for individual multiple comparisons. Error bar: standard deviation (SD). Data are mean ± SD. * P < 0.033, ** P < 0.002 and *** P < 0.001.

Article Snippet: Primary Bone Marrow CD34+ Hematopoietic Cells, Normal, Human were acquired from ATCC (PCS-800-012).

Techniques: High Throughput Screening Assay, Expressing, Western Blot, Immunoprecipitation, Construct, Control, Agarose Gel Electrophoresis, Negative Control, Standard Deviation

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 1-month-old goat testis. ( A-C ) DIF of CD34/α-SMA in 1-month-old goat testis; ( D-F ) DIF of CD34/α-SMA in blood vessels of 1-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); CD34 + cells (white triangular arrows); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-C ):10 μm; ( D-F ): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 2-month-old goat testis. ( A-C ) DIF of CD34/α-SMA in 2-month-old goat testis; ( D-F ) DIF of CD34/α-SMA in blood vessels of 2-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); CD34 + cells (white triangular arrows); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-C ): 20 μm; ( D-F ): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 12-month-old goat testis. ( A-I ) DIF of CD34/α-SMA in 12-month-old goat testis; ( J-L ) DIF of CD34/α-SMA in blood vessels of 12-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-F, J-L ): 10 μm; ( G-I ): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 1-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 1-month-old goat testis. (1-a), (1-b), (1-c) are respectively enlarged images of (1) in ( A ), ( B ), ( C ). (2-a), (2-b) and (2-c) are the enlarged images of (2) in ( A ), ( B ) and ( C ) respectively. CD34 (red); α-SMA (green); DAPI (blue); TC (yellow, indicated by white triangular arrows); ST. Seminiferous tubule; SC. Sertoli cells. Scale bar = ( A, B, C ): 20 μm; (1 - a) - (1 - c), (2- a) - (2 - c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 2-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 2-month-old goat testis. (a-c) are enlarged views of the boxed areas in ( A-C ). CD34 (red); α-SMA (green); DAPI (blue); TC (yellow, indicated by white triangular arrows); ST. Seminiferous tubule. Scale bar = ( A-C ): 20 μm; (a-c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. CD34 (red); Vimentin (green); DAPI (blue); ST. Seminiferous tubule; S. sperm; PS. Primary spermatocyte; SC. Sertoli cells; RS. Round sperm cells; White triangular arrows. Peritubular myoid cell nucleus. Scale bar = ( A-C ): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. (a-c) is an enlarged view of the white dotted box area of ( A-C ). CD34 (red); Vimentin (green); DAPI (blue); TC (yellow, indicated by white triangular arrows). Scale bar = ( A-C ), (a-c): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. (1-a), (1-b), (1-c) are respectively enlarged images of (1) in ( A-C ). (2-a), (2-b), (2-c) are the enlarged images of (2) in ( A-C ). CD34 (red); Vimentin (green); DAPI (blue); TC (yellow); White triangular arrows. Peritubular myoid cell nucleus. Scale bar = ( A-C ): 50 μm; (1-a) - (1-c), (2-a) - (2-c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Translational Cancer Research

Article Title: The suppressing effects of VEGF-mediated angiogenesis at different administration sequences of apatinib and transarterial embolization in vivo

doi: 10.21037/tcr.2019.12.97

Figure Lengend Snippet: Immunohistochemical staining showed a positive CD34 reaction (×200). Positive CD34 was identified by dark-brown staining. (A) Control group (CG); (B) combined Group 1 (CG1); (C) combined Group 2 (CG2); (D) combined Group 3 (CG3).

Article Snippet: Both rabbit anti-rat VEGF-165 polyclonal antibody and rabbit anti-rat CD34 polyclonal antibody were purchased from BOSTER, Inc, Wuhan, China.

Techniques: Immunohistochemical staining, Staining, Control

Journal: Nature Genetics

Article Title: Cell-type-specific consequences of mosaic structural variants in hematopoietic stem and progenitor cells

doi: 10.1038/s41588-024-01754-2

Figure Lengend Snippet: a , Circle-packing plot of mSVs found in BM712. b , Strand-seq karyograms of unmutated (WT; upper), 17p-Del (somatic mosaic heterozygous deletion on chromosome 17p) only (middle) and 17p-Del and 17q-Del (somatic mosaic heterozygous deletion on chromosome 17q) (bottom) somatic genotypes in single cells. c , Bubble hierarchy plot of mSVs identified in BM712. Bubbles are colored by somatic genotype, and scaled proportionally to each subclone’s frequency within the donor. CF is noted beside each bubble, and the distinguishing mosaicism acquired by each subclone indicated on the adjoining arm from the parent population. d , e , UCSC genome browser tracks for the 17p-Del ( d ) and 17q-Del ( e ) genomic segments. Tracks for both panels include composite read data and BreakpointR -based breakpoint assignments, and highlight relevant genes. In e , the high-confidence deletion call from bulk WGS is also displayed (VAF inferred by Delly2 (ref. ) is 28.5%). f , Heatmap of genes showing differential NO between WT, 17p-Del and 17pq-Del cells. g , Pathway over-representation analysis using ConsensusPathDB for the genes identified in the pairwise comparison of 17p-Del and 17pq-Del subclones with WT cells (FDR 10% based on the hypergeometric test). On the x axis, Act_U and Act_D depict increased and decreased activity, respectively. h , UMAP plot of scRNA-seq of CD34 + cells, with inferred cell type from reference data overlaid. i , Cell-type composition and enrichment analysis for 17p-Del and 17pq-Del subclones in scRNA-seq data of CD34 + cells. Asterisks indicate cell types with significant enrichment in a given subclone, based on Benjamini–Hochberg-adjusted Fisher’s exact test. j , UMAP plot of scRNA-seq of CD34 − cells, with cell type inferred from single-cell reference datasets , overlaid. k , Cell-type composition and enrichment analysis for the 17p-Del subclone in scRNA-seq of CD34 − cells. ‘Unresolved’, the 17q-Del subclone could not be resolved in these scRNA-seq data owing to the low numbers of expressed genes covered. Significant cell-type enrichment with ** P adj < 0.001 or *** P adj < 0.0001, respectively, based on two-sided Fisher’s exact test followed by Benjamini–Hochberg multiple testing correction. In CD34 + cells, CMPs and LMPPs are enriched in the 17p-Del subclone ( P adj = 1.99 × 10 −11 and P adj = 6.48 × 10 −3 , respectively) and HSCs are enriched in the 17q-Del subclone ( P adj = 2.07 × 10 −5 ). In the case of CD34 − cells, monocytes are enriched in the 17p-Del subclone ( P adj = 9.6 × 10 −29 ). dups, duplications; NK, natural killer.

Article Snippet: For each donor, two samples were prepared: one sample of CD34 + cells and one sample a 50:50 mixture of CD34 + and CD34 − cells. scRNA-seq libraries for each sample were generated as per the standard 10X Genomics Chromium 3′ (v.3.1 chemistry) protocol.

Techniques: Comparison, Activity Assay